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In 2019, Daniela and others from the Autonomous State University of Nuevo Leon, Mexico, studied the antibacterial and anti-biofilm effects of neutral electrolyzed water (hypochlorous acid as the main component) on Helicobacter pylori. The researchers used a product called OxOral® Antibacterial mouthwash, pH value is 6.4-7.5, the concentration of active substance (the main component is HOCl) is 15 ppm, and the oxidation-reduction potential (ORP) is 650-900 mV.
Studies have shown that in the dose range of 15 ppm to 3.75 ppm, the antibacterial activity of OxOral® mouthwash has no difference (p>0.05) compared with 5μg/mL tetracycline (antibiotic), that is, the two effects are equivalent. Therefore, a 3.75 ppm OxOral® mouthwash (inhibition rate of 99.7±7.7%) was determined as the MIC (minimum inhibitory concentration) value. In addition, residual inhibition was observed at 1.875 ppm and 0.938 ppm, and the inhibition rates were 81.1±6.0% and 53.3±2.8%, respectively (as shown in the figure below). When no visible microbial growth is observed, the MBC (Minimum Bactericidal Concentration) value is 7.5 ppm.
However, when the biofilm cells were treated with 0.938 ppm, 0.469 ppm and 0.234 ppm OxOral® mouthwash, the antibacterial rates reached 99.9±5.5%, 89.1±1.2% and 35.5±0.9%, respectively (as shown in the figure below); 5μg/mL The bacteriostatic rate of tetracycline reaches 100±13.5%. Compared with the normal saline control group, no difference was observed in OxOral® mouthwash with a concentration of 0.117ppm (p>0.05).
The cytotoxicity test of OxOral® mouthwash showed that compared with the DMEM control group (no cytotoxicity), no significant difference was observed in the concentration range of 0.469 ppm to 15 ppm (p>0.05), showing OxOral® mouthwash Security. The result is shown in the figure below:
When the effective chlorine concentration is 7.5ppm-15ppm, hypochlorous acid has a good bactericidal effect on Helicobacter pylori;
When the effective chlorine concentration is greater than 3.75ppm, hypochlorous acid has a good bacteriostatic effect on Helicobacter pylori;
When the available chlorine concentration is greater than 0.938 ppm, hypochlorous acid has a good inhibitory effect on Helicobacter pylori biofilm;
When the effective chlorine concentration is within 15 ppm, hypochlorous acid does not show cytotoxicity and has high safety.
This study shows that a mouthwash with hypochlorous acid as the main functional component has high antibacterial and anti-biofilm effects and low cytotoxicity against Helicobacter pylori. The result of the study is that hypochlorous acid is used as a routine treatment for patients with Helicobacter pylori infection Supplementary drugs provide the possibility.
